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The distinction matters enormously: it is the difference between exposing cells to a free synthetic peptide of uncertain stability and having living tissue produce the E-domain in its natural molecular and cellular context

These tissue sections were deparaffinized in graded xylene, rehydrated in graded alcohol solutions, and then incubated in antigen retrieval buffer (Roche, Basel, Switzerland) at 37 C for 30 min

Basturk A, Isik I, Atalay A, Yilmaz A

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Your body's ability to process and utilize vitamin B12 can be influenced by genetics, diet, and underlying health conditions